Products 1 – 7 of 7 Receipt sugar cookies, Grand masti collection report standard, practice form answer key, Bd bbl enterotube ii interpretation guide. Remarks: Glucose – Any degree of yellow is positive. Acid end products from glucose fermentation turn the pH indicator from red (alkaline) to yellow (acid). BD Micro, GUIDE ENTEROTUBE II INTERPRETATION GUIDE, Enterotube, Each. BD Micro, GUIDE ENTEROTUBE II INTERPRETATION GUIDE, Enterotube.

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If no color change, or interrpetation orange color, is observed in the glucose compartment and growth is evident by color changes in other compartments, the organism is not a member of the family Enterobacteriaceae. All organisms require a supply of energy and matter to build themselves and to continue to function.

Coli Microaerophile Growth throughout. Manual of clinical microbiology, 8 th ed.

This is due to the fact that there are only a few basic shapes and physical features More information. For professional use only Do not use tubes if they show evidence of microbial contamination, discoloration, wax detachment from the respective media, drying, cracking or other signs of deterioration.

Biol Lab Manual: Interpretation of Enterotube II

In this investigation, you will study several More information. This is a group activity. This test uses a More information. To study chemical and physical properties of proteins from natural sources egg and milk and some chemical reactions of amino acid residues in these proteins, More information.

If positive, circle the appropriate numbers on the prepared sheet. Taxa other than those listed in Table 3 are not intended for use in this system. Bacterial fermentation of dulcitol, which results in the formation of acidic end products, is indicated by a change in color of the indicator present in the medium from green alkaline to yellow or pale yellow acidic.



Using the Enzyme worksheet and Enzyme lab handout answer the Pre-Lab questions the pre-lab must be complete before beginning the lab.

Nahaisi Abstract This study was conducted to investigate the.

The urease test is strongly positive for Proteus species and may be evident as early as 4 to 6 h after incubation; it is weakly positive light pink color lnterpretation 18 to 24 h incubation for Klebsiella and Enterobacter species. Identification of Unknown Organic Compounds Identification of Unknown Organic Compounds Introduction The identification and characterization of the structures of unknown substances are an important part of organic chemistry.

Urine Culture Diagnostic Techniques: Outer membrane of cell wall Lipid A What is it? Cellular Respiration, Fermentation and Photosynthesis Introduction: GRAM negative pathogens cocci and bacilli. All other tests must be read before the indole and Voges-Proskauer tests are performed as the reagents added for these tests may alter the remainder of the BBL Enterotube II reactions. The VP test is mandatory only if the database with VP is used.

INSTRUCTIONS FOR USE BBL Enterotube II. IA Rev.: Jan. 2007

We proposed in the Basic Microbiology More information. All reactions which should be positive may be of equal, greater or lesser intensity than the colors indicated in the Color Reaction Chart.

When preparing and handling indole and VP guie, observe appropriate handling instructions and Risk and Safety Phrases. Do not flame wire.


The tip of the inoculating wire is under the white cap. Explain how to sterilize it, More information. What does it mean when More information. The culture used for the inoculation should be at least 18 hours old, but should generally not be older than 48 hours. Instruct students to put on lab coats and gloves; clean their benches. As we shall explore in this lab exercise, More information. Gelatin Hydrolysis Test Protocol The Gelatin Hydrolysis Test is used to detect the ability of microorganisms to produce the enzyme gelatinase.


Enzyme Pre-Lab Using the Enzyme worksheet and Enzyme lab handout intrrpretation the Pre-Lab questions the pre-lab must be complete before beginning the lab. If this medium remains red after inoculation and incubation, the strain does not belong to the Enterobacteriaceae. Provided is a listing of the genus, species, number of strains tested and the percent of organisms giving positive biochemical reactions. Start display at page:. The hydrogen sulfide reacts interpretqtion the iron salts also present in the medium to form a black precipitate of ferric sulfide usually along the line of inoculation.

Selective media allow certain types of organisms to grow, and inhibit the growth More information. They are catalase positive and nearly all of them are oxidase negative [It has been proposed recently to include Plesiomonas shigelloides into the Enterobacteriaceae based on 16S rrna sequencing and because it contains the enterobacterial common antigen.

The portion of the wire remaining in the tube maintains anaerobic conditions necessary for true fermentation of glucose, production of as and decarboxylation of lysine and ornithine. Disseminated Intravascular More information. The presence of acetoin is indicated by the development of a red interpretatiln within 5 to 20 min.

The medium may become a deeper shade of green after incubation. Explain how to sterilize it. Tubes from opened packages can be used up to the ii date. This test detects those organisms which are capable of utilizing citrate, in the form of its sodium salt, as the sole source of carbon. Add drops of Kovacs reagent cat. Over all replicates, agreement in identification was Media, incubation, and aseptic technique Objectives 1.